m komossense atcc 33013 t  (ATCC)

Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A–F) Static time kill experiments represent the change in the viable count of M. tuberculosis (closed squares) and M. komossense (open circles) in response to exposure to isoniazid (H) at the displayed multiple of MIC [1 mg/L (concentrations used-0.25–8 mg/L)].

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques:

Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A–F) Static time kill experiments represent the change in the viable count of M. tuberculosis (closed squares) and M. komossense (open circles) in response to exposure to rifampicin (R) at the displayed multiple of MIC [0.4 mg/L (concentrations used- 0.1-3.2 mg/L)].

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques:

Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A,B) Change in the viable count of M. komossense in the hollow fiber system in response to rifampicin alone or in combination with isoniazid at the displayed multiples of MIC. Data displayed are mean values of three technical replicates.

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques:

Journal: Frontiers in Antibiotics

Article Title: Implications of drug-induced phenotypical resistance: Is isoniazid radicalizing M. tuberculosis ?

doi: 10.3389/frabi.2022.928365

Figure Lengend Snippet: (A) Nile red staining of M. komossense of bacteria harvested from a 168 h hollow fiber experiment where bacteria were exposed to isoniazid at 1 × MIC (1 mg/L) Green flourescence indicates the presence of one or more lipid bodies in the bacterial cells. Red fluorescence is the result of the stain interacting with the polar lipids in the bacterial outer envelope. Individual samples of bacteria were defined as S= susceptibile or R= resistant based on a microbroth dilution suceptibility test conducted agaisnt isoniazid, rifampicin, ethambutol, pretomanid, and bedaquiline. (B) Lipid body staining of M. tuberculosis was achieved with lipophillic Nile red. Green flourescence indicates the presence of one or more lipid bodies in the bacterial cells. Red fluorescence is the result of the stain interacting with the polar lipids in the bacterial outer envelope. Individual samples of bacteria were defined as S = susceptibile or R = resistant based on a microbroth dilution suceptibility test conducted agaisnt isoniazid, rifampicin, ethambutol, pretomanid, and bedaquiline.

Article Snippet: Isolates of M. komossense (ATCC 33013), a rapid growing hazard group 1 organism and M. tuberculosis (H37Rv, NCTC 7416) were incubated in sealed 50 ml tubes (Falcon, Corning, USA) with Middlebrook 7H9 (Fluka) with 0.05% Tween (Sigma Aldrich) at 30°C for 3–5 days or at 37°C for 28 days until confluent or at ≥0.1 OD 600 .

Techniques: Staining, Bacteria, Fluorescence

Journal:

Article Title: Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR-Restriction Enzyme Analysis of hsp65

doi: 10.1128/JCM.40.11.4191-4196.2002

Figure Lengend Snippet: Restriction patterns of 43 reference isolates from 34 Mycobacterium species

Article Snippet: We also determined restriction profiles for M. chelonae subsp. niacinogenes , M. chubuense , and M. komossense , species for which no identification patterns have been reported earlier (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Species Strain a Pattern b M. africanum UG MB3 M. tuberculosis complex I M. agri ATCC 27406 235/130/85-160/145/60 M. aichiense ATCC 27280 320/115-195/70/60 M. asiaticum ATCC 25276; RIVM {"type":"entrez-nucleotide","attrs":{"text":"Z26114","term_id":"398680","term_text":"Z26114"}} Z26114 M. asiaticum I M. aurum ATCC 23366 441-125/115/70/60 ATCC 23366 320/115-140/95/60/55 M. avium ATCC 25291 M. avium I M. bovis ATCC 19210 M. tuberculosis complex I M. bovis BCG-Moreaux ATCC 35736 M. tuberculosis complex I M. chelonae NCTC 946; UG MB41 M. chelonae I + 60 M. chelonae subsp. niacinogenes ATCC 19237 320/115-175/145 M. chitae ATCC 19627 320/115-140/85/60 M. chubuense ATCC 27278 235/205-140/85/60 M. diernhoferi ATCC 19340 320/115-145/140/60 M. flavenscens ATCC 14474 M. flavescens I + 60 M. fortuitum ATCC 6841 M. fortuitum I + 60/55 M. gordonae ATCC 14470 M. gordonae I M. gordonae ureolyticum RIVM MIS222 M. gordonae III M. intracellulare ATCC 13950 M. intracellulare I M. kansasii ATCC 12478 M. kansasii I M. komossense ATCC 33013 320/115-145/140/60 M. mageritense UAM M. mageritense I M. marinum ATCC 927; RIVM MIS 14 M. marinum I M. neoaurum ATCC 25790 M. neoaurum I -55 M. obuense ATCC 27023 235/205-140/85/60 M. phlei ATCC 11758 M. phlei I M. porcinum ATCC 33776 235/205-140/125/95/55 M. rhodesiae ATCC 27024 320/115-160/125/60 M. simiae ATCC 25275 M. simiae I M. smegmatis ATCC 19420 M. smegmatis I M. smegmatis mc 2 155 Pasteur Institute M. smegmatis I M. szulgai NCTC 10831 M. szulgai I M. terrae ATCC 15755 M. terrae I M. thermoresistibile ATCC 19527 235/205-175/135/70 M. tokaiense RIVM T 47502 235/130/85-140/95/80/60 M. triviale ATCC 23292 M. triviale I M. tuberculosis H37Rv ATCC 27294 M. tuberculosis complex M. tuberculosis H37Ra ATCC 25177 M. tuberculosis complex M. vaccae ATCC 15483 M. vaccae I M. xenopi RIVM MYC527 M. xenopi I Open in a separate window a UG, University of Ghent, Ghent, Belgium; ATCC, American Type Culture Collection, Manassas, Va.; RIVM, National Institute of Public Health and the Environment, Bilthoven, The Netherlands; NCTC, National Collection of Type Cultures; CRPHF, Reference Center for Tuberculosis Professor Hélio Fraga, Rio de Janeiro, Brazil; UAM, Autonomous University of Madrid. b The numbers represent the molecular sizes (in base pairs) of the bands.

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